Effect of New Ceramic Materials Based on Lanthanum Zirconate on Human Cell Culture
No. 4 (2023), Articles
No. 4 (2023)

Effect of New Ceramic Materials Based on Lanthanum Zirconate on Human Cell Culture

Articles
Published 27.12.2023
Elena A. Volokitina
Ural State Medical University (Ekaterinburg, Russia); Institute of High Temperature Electrochemistry of the Ural Branch of the Russian Academy of Science, (Ekaterinburg, Russia
Maria V. Ulitko
Ural Federal University named after the first President of Russia B. N. Yeltsin (Ekaterinburg, Russia)
https://orcid.org/0000-0003-3193-2903
Yulia Ya. Antonets
Ural Federal University named after the first President of Russia B. N. Yeltsin (Ekaterinburg, Russia)
Irina P. Antropova
Ural State Medical University (Ekaterinburg, Russia); Institute of High Temperature Electrochemistry of the Ural Branch of the Russian Academy of Science, (Ekaterinburg, Russia)
https://orcid.org/0000-0002-9957-2505
Kirill A. Timofeev
Ural State Medical University (Ekaterinburg, Russia); Institute of High Temperature Electrochemistry of the Ural Branch of the Russian Academy of Science, (Ekaterinburg, Russia)
PDF (Русский)

Keywords

bone tissue defects
augmentation
bone replacement material
bioceramics
lanthanum zirconate

Funding data

Abstract

Bone defects are becoming increasingly common and have a huge impact on the quality of life of patients. The development of new materials for bone implantation is a major challenge. Оbjective of the study is to determine the influence of new ceramic materials based on lanthanum zirconate on human cell culture in order to assess the prospects for their use as bone replacement implants. Materials and methods. To study the cytocompatibility of the new materials, a culture of human dermal fibroblasts was used. Bioceramic samples: La2Zr2O7 (undoped lanthanum zirconate, LZ), La0.9Ca0.1Zr2O6.95 (calcium-doped lanthanum zirconate, LZCa) and La0.9Sr0.1Zr2O6.95 (lanthanum zirconate doped with strontium, LZSr), were used in the form of round plates (d = 5 mm, h = 2 mm). Sterile plates were placed into the wells of a 24-well plate and a cell suspension (500 μl) was added. Cells were cultured without changing the medium for 5 days. Control — wells without bioceramic samples. The cytocompatibility study included determination of the viability and proliferative activity of cells after 1, 3 and 5 days of cultivation. Results. The study determined the cytocompatibility of the studied ceramics based on lanthanum zirconate. An increase in the proliferative activity and viability of human fibroblasts after a period of cell adaptation was shown. It has been established that doping lanthanum zirconate with calcium or strontium slows down the adaptation of human fibroblasts during cultivation without significant differences between LZCa and LZSr. Conclusions. The viability of human fibroblasts varies within acceptable values and is sufficient to maintain their recovery potential when growing on the ceramic materials under study. The implementation of this stage of preclinical research provides justification for further study of these materials for bone tissue augmentation.

Funding.
The work was supported by the Russian Science Foundation (grant No. 22-25-20037), https://rscf.ru/en/project/22-25-20037/.

For citation
Volokitina EA, Ulitko MV, Antonets YuYa, Antropova IP, Timofeev KA. Effect of new ceramic materials based on lanthanum zirconate on human cell culture. Bulletin of USMU. 2023;(4):7–17. (In Russ.). EDN: https://elibrary.ru/MIPNBM.

PDF (Русский)

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Copyright © 2023 Volokitina E. A., Ulitko M. V., Antonets Yu. Ya., Antropova I. P., Timofeev K. A.