Supplementary Files
Keywords
children
intestinal microbiota
dysbiosis
Categories
Abstract
Introduction. Violation of the intestinal barrier in dysbiosis affects the course of immunopathological processes in the body, which is a factor in the development of many diseases, including atopic dermatitis (AtD).
Objective is to analyze the composition of the intestinal microbiota in children 1–7 years old with AtD and determine the relationship between the severity of dysbiotic disorders and the severity of the clinical course of the disease.
Materials and methods. We observed 55 children 1–7 years (24 boys and 31 girls) suffering from AtD of varying severity. All patients underwent a collection of complaints and medical history, a physical examination, an assessment of the severity of AtD on the SCORAD scale, and an assessment of the state of intestinal microbiocenosis using culture and mass spectrometric methods.
Results. A decrease in the number of bifidobacteria (< 108 CFU/g) was noted in all 55 patients (100 %), lactobacilli (< 106 CFU/g) — in 36 children (65 %). A decrease in the content of Escherichia coli with normal enzymatic activity was detected in 41 children (75 %). Lactose-negative E. coli was detected in 16 patients (29 %). Staphylococcus aureus was isolated in 16 patients (29 %), and its frequency increased with increasing severity of AtD: 3 patients (15 %) with mild, 5 (25 %) with moderate, and 8 children (53%) with severe (p = 0,06 for comparison of groups with mild and severe degrees). Yeast-like fungi of the genus Candida were found in 20 patients (36 %). The most significant difference was noted when comparing groups of mild and severe degrees: Candida spp. It was detected in 3 (15 %) children with mild and in 8 (53 %) with severe severity (p = 0.04). Opportunistic bacteria Ruminococcus spp. and Citrobacter spp. They were isolated in 28 (51 %) and 20 (36 %) patients, respectively, but their frequency did not significantly differ depending on the severity of AD (p > 0,05).
Conclusion. The revealed qualitative and quantitative changes in the intestinal microbiota in children with severe AtD necessitate further in-depth study of the mechanisms of interaction between the intestinal microbiota and immune-dependent skin inflammation, which will allow us to identify possible application points for predicting the severity of AtD in children.
For citation: Kirichenko OV, Ivanova EV, Popova LY, Alemanova GD, Chaynikova IN. Characteristics of the intestinal microbiota in children with atopic dermatitis of varying severity. USMU Medical Bulletin. 2026;11(2):e00220. (In Russ.). DOI: https://doi.org/10.52420/usmumb.11.2.e00220. EDN: https://elibrary.ru/CHYVVK.
Funding. The work was completed without attracting additional funding.
Conflict of interest. The authors declare that there is no conflict of interest.
Ethics statement. The study protocol was approved by the Local Ethics Committee of the Orenburg State Medical University (No. 363 dated 12 September 2025). All patients gave voluntary informed consent to participate in the study and publish its results in an impersonal form. The study was conducted in accordance with the provisions of the Declaration of Helsinki (as amended in 2024).
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Copyright © 2026 Kirichenko O. V., Ivanova E. V., Popova L. Y., Alemanova G. D., Chaynikova I. N.